Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc TAE,10X (TRIS ACETATE EDTA)4L
TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. 10X concentrated ready to be diluted to a 1X working solution. One 4 liter cubitainer.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
Mitegen Tris/ HCl, 1M, pH8.5, 100 ml, CAS # 77-86-1
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Boca Scientific Inc Antibody Stabilizer PBS Base, 50ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
Sigma Aldrich Fine Chemicals Biosciences MES Hydrate | 1266615-59-1 | MFCD00149409 | 1kg
MES Hydrate | Purity: 99% | Mol Wt: 195.24 (anhydrous basis) | 1266615-59-1 | MFCD00149409 | 1kg
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X, for Western Blot Washing) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Boca Scientific Inc Coating Buffer, pH 7.4, Based on PBS, 500ml
Coating Buffer is designed for adsorptive immobilization of proteins and antibodies on plastic surfaces, such as microtiter plates, or other protein binding surfaces. Depending on the protein or the antibody, the pH of the Coating Buffer is crucial for efficient immobilization, as the pH-value can have an influence on the steric structure of proteins or antibodies. Coating Buffer is offered with a pH of 7.4, based on PBS, or 9.6, based on carbonate. For the optimization of the coating procedure of a newly developed immunoassay, we strongly recommend testing the two Coating Buffers side by side. Depending on the type of surface and the type of proteins or antibodies, the required incubation time can vary.
Medchemexpress LLC PBS-T Powder | 100 POUCHES
MCE PBS-T Powder (1 L of 1x) is a formulation consisting of NaCl, KCl, Phosphate, and Tween 20. It is extensively utilized as a washing buffer in immunoassay experiments such as ELISA and Western Blot. Additionally, it can be employed for antibody dilution and the preparation of blocking solutions. The product is conveniently packaged, with 20 pouches defined as the base specification, and larger sizes offered as incremental volumes of this base. Each pouch is designed to be diluted to 1 L of 1x PBS-T buffer.
- Widely used in ELISA and Western Blot.
- Can be used for antibody dilution and preparation of blocking solution.
- Consists of NaCl, KCl, Phosphate, and Tween 20.
- Each pouch dilutes to 1 L of 1x PBS-T buffer.
- Eliminates the need to weigh and mix individual components.
- Convenient way to make PBS-T buffer.
- Final 1x PBS-T buffer has a pH of 7.4 ± 0.2 @ 25°C.
Alkali Scientific CellPro™ MOPS Buffer 10X, Amber Bottle, 1L
Alkali Scientific™ CellPro™ MOPS Buffer 10X is a versatile reagent commonly used in molecular biology and biochemistry applications. With its optimized formulation, this buffer provides stable pH conditions, crucial for nucleic acid electrophoresis, RNA isolation, and protein gel electrophoresis. Each batch undergoes stringent sterile filtration to ensure purity and consistency, making CellPro™ MOPS Buffer 10X a trusted choice for researchers seeking reliable results in their experiments.
Alkali Scientific CellPro™ Tris-HCL, 1M, Stock Solution, pH 9.0, 1000mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ Tris Glycine SDS Buffer 10X, pH 8.3, 1L
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Alkali Scientific CellPro™ Tris-HCl Buffer, 1M, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ PBS 10X, pH 8.0, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.